Gas-liquid two-phase flow is used to simulate double bubble collision process numerically with LES method, fluid volume method, crushing criterion and the third-generation vortex identification method. The single variable method is used to study the influence of bubble diameter ratio, relative eccentricity and relative distance between bubbles on the degree of bubble fragmentation. In the process of double bubble collision, the closer the diameter of the two bubbles is, the weaker the bubble breaking degree is. The relative eccentric distance has little effect on the bubble breaking degree. It shows that as the relative distance is less than 1, the bubble breaking degree is more obvious with the increase of the relative distance. As the relative distance is more than 1, the bubble breaking degree tends to be flat. It also shows that the third-generation vortex identification method captures the vortex position in the two-phase turbulent flow field well, and reflects sensitively the turbulent changes.